SIGMAKOKI

Application System
Bio-photonics
Catalog Code:W2087
Localized Laser Heating System for Infrared Laser-Evoked Gene Operator Systems (IR-LEGO)

Low cost is achieved by commonizing the parts with Optical Tweezers Mini2 except for the laser light source.

▶ The infrared laser (1462nm) can directly heat water in the biological region.
▶ Local heating method by single-point irradiation using a microscope.
▶ Can be connected to existing inverted and upright microscopes. (Please refer the diagram)
▶ A laser light source with red guide light is added for checking the laser irradiation position. (*1)

What is IR-LEGO ?

Infrared Laser-Evoked Gene Operator (IR-LEGO) is developed as the world’s first technology by consolidated research team led by Dr. Shunsuke Yuba at National Institute of Advanced Industrial Science and Technology (AIST). This is a technique to induce specified genes that are under the control of a heat shock promoter at defined time, by heating single cells that consist of genetically-modified organisms, with an infrared laser. IR-LEGO could be adopted to all the genetically-modified experimental organisms that the heat shock promoters function and the internal focus of infrared laser are available. Because of its high efficiency and reproducibility, and less detrimental effect from laser, IR-LEGO is a new and prospective tool for future gene function analysis.

Applications of IR-LEGO for Various Species

Utilizing a strain (cell) that carries a heat shock promoter driven transgene, an infrared (IR) laser is irradiated at parts indicated by white arrowhead marks.


Example of induced RFP expression by IR laser irradiation on the GFP marked neuron of the nematode (C. elegans) The white arrow indicate a neuron not irradiated.
The red flourescence by RFP is obtained on a neuron and neuraxon that irradiated by a laser (white arrowhead).

[Photo credit] Dr. Motoshi Suzuki & Dr. Shin Takagi, Nagoya University

Example of induced GFP expression by IR laser irradiation on a pineal gland of medaka (O. latipes) larvae.

[Photo credit]
Dr. Tomonori Deguchi, National Institute of Advanced Industrial Science and Technology (AIST)
Dr. Yasuhiro Kamei, National Institute for Basic Biology (NIBB)

Example of induced Kaede expression by IR laser irradiation (2 points) on a part of zebrafish (D. relio) retina.
Kaede is partially photoconverted after its expression (purple arrow).

[Photo credit] Dr. Mariko Itoh & Dr. Kohei Hatta, University of Hyogo

Example of induced GUS expression by IR laser irradiation on lateral root tips of Arabidopsis (A. thaliana).

[Photo credit] Dr. Hiroko Urawa & Dr. Kiyotaka Okada, National Institute for Basic Biology (NIBB)

Result of Gene Expression Check Test


[Photo credit]
Dr. Shunsuke Yuba, National Institute of Advanced
Industrial Science and Technology (AIST)
Dr. Yasuhiro Kamei, National Institute for Basic Biology (NIBB)

Example of temperature rise after single-point irradiation
of an aqueous solution with an infrared laser (λ=1462nm)
Example of laser irradiation on a black paint coated surface
(using a 20x objective lens)
【Point】

▶ Conditions for temperature rise varies depending on the laser power,
the irradiation time, etc.
▶ Select an objective lens with high transmittance at the laser wavelength.
▶ Exact irradiation conditions can be checked using a power meter (sold separately).

【Point】

▶ The beam diameter of the red guide light differes from that of the infrared
laser due to chromatic aberration.
▶ Red guide light is an auxiliary light for rough irradiation position confirmation.

Attention

The position of the laser beam is fixed at the center of the microscope's field of view.

This is a direct connection to the microscope, so the mounting adapter may vary depending on the model of the microscope. Please refer to the product diagram below to select a product.

In order to connect to microscopes made by other manufacturers, a turret for mounting a fluorescent mirror cassette is required. In addition, the use of a hierarchical structure (two-stage structure) is required for use with fluorescence observation (upper stage: laser, lower stage: light source for fluorescence).

To construct the system to a microscope, an assembly and adjustment fee will be required separately. Please contact our sales with the information about the manufacturer and part number of the microscope you are using.

Product Diagram

*1 LMS2 allows to connect to the core unit products or cage systems via CU-011. Compatible with constructed core unit microscope such as CUS-BF, CUSmini-BF, etc.
*2 Compatible with Ti2 series and Ti series microscopes of Nikon.
*3 Compatible with IX83 and IX73 inverted microscopes or BX series upright microscopes of Olympus. Select LMS2-AD-OL-IX to connect with inverted type and LMS2-AD-OL-BX
to connect with upright type.
*4 LMS2 may not be able to be connected depending on the options and installation environment attached to the microscope even with the above compatible models.
Please contact our sales for details.
*5 Please contact our sales for support of microscopes other than the supported models above.
*6 The laser focusing position difference due to chromatic aberration of each objective lens can be corrected with the motorized focus.